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Luciferase-labeled cell lines: a valuable tool for oncology studies

Author: Manali Dimri, PhD | Scientist, Scientific Development
Date: July 2021


Luciferases are oxidative enzymes that emit light in the presence of a substrate (D-luciferin) within a living organism, a process known as bioluminescence. The gene for the most common luciferases comes from the family of light producing enzymes called firefly luciferases1, 2

In preclinical research, small animal in vivo imaging plays an essential role in visualizing physiological processes, progression of disease and development of therapies. Luciferase (luc) enabled cell lines offer a simple, high-throughput and robust means to quantitatively assess tumor burden and response of tumors to treatment therapies in subject animals, through bioluminescence imaging (BLI)1, 2. Click here to learn more about BLI

The sensitivity and accuracy of BLI systems offers multiple advantages over traditional methods, such as:

(1)    Noninvasive real-time whole-body in vivo tumor monitoring and imaging

(2)    Continuous assessment of tumor progression and response to therapeutic treatments in the same animal

(3)    Metastasis assessment

(4)    Reduced need for animal sacrifice

Why Labcorp Drug Development (formerly Covance Laboratories)?

Covance was the first Contract Research Organization (CRO) to offer BLI, in 2003, and in the past 18 years we have accrued extensive experience in this optical imaging field. We offer a large panel of luciferase-enabled cell lines with over 80 unique hematological malignancy cell lines (Table 1). We have a dedicated team of experts to design the best oncology study for you as well as to ensure smooth study execution. Our scientists are skilled to engineer our in-house cell lines or your cell line of interest as well as to custom make vectors to express luciferase for BLI detection. Complete list of cell lines

LabCorp Drug Development (formerly Covance Laboratories) has a license agreement from Dana Farber Cancer Institute and other organizations that provides additional access to many characterized, in vivo validated luciferase-expressing tumor lines.

Our luciferase-expressing tumor cell lines have

·       Stable luciferase expression

·       A fluorescent protein (mCherry) along with the Luc 2 gene

·       Quantitative correlation between signal strength and cell numbers

·       High sensitivity and low signal-to-noise ratio

·       Availability of multiple tumor cell lines from human, mouse, and rat

·       Suitable for in vitro as well as for in vivo assays

We also offer an alternative method for cell transduction in luc- cell enabling which is cell electroporation allowing clients to choose customized vectors instead of a lentiviral vector.

Service offerings related to BLI include:

How it works?

Typically, cancer cells are engineered to express the firefly luciferase gene along with a puromycin resistance gene using a lentiviral system for transduction (Fig. 1A). Along with the Luc 2 gene, the construct has a fluorescent protein coding gene (mCherry) that enables the detection of tumor cells in different tissues over time. Cells are cultured in the presence of puromycin to select the cells with inserted lentiviral vector encoding firefly luciferase (Fig. 1B). Light output is generated (Fig. 1C(ii)) from the luciferase enabled cells and bioluminescence is measured using the IVIS® In Vivo Imaging System (Fig. 1C (i)); Luc-enabled cells are then engrafted into mice to form tumors. Following the injection of D-luciferin (substrate), the luciferase enzyme will catalyze this substrate resulting in light emission detected with IVIS® (PerkinElmer, Waltham, MA) (Fig. 1D(i)) and analyzed in regions of interest using the Perkin Elmer's Living Image software (Fig. 1D (ii)).

Fig. 1: Representation of generation of luciferase-expressing tumor cell lines for in vivo bioluminescence imaging. A. Tumor cell lines are transduced with a lentivirus vector. B. Transduced tumor cell lines are selected and expanded using selection marker. C. (i) Raji cells were transduced with lentiviral vector and luciferase expression was confirmed upon addition of D-luciferin substrate. (ii) Luciferase bioluminescence was quantified using Perkin Elmer's Living Image software and number of cells vs total flux (p/s= photons/second) was plotted. D. (i) Monitoring tumor burden using BLI. Luminescence imaged using IVIS® at different time points. (ii) Luciferase bioluminescence was quantified using the Perkin Elmer's Living Image software.

Histotype

Cell line

Espèces

Cerveau

D54-Luc

Homme

 

Gli36-DsRed-R-Luc (récupéré)

Homme

 

LN-827(pMMP-LucNeo)

Homme

 

U-251-Luc-mCh-Puro

Homme

 

U-87 MG-Luc

Homme

 

GL261-Luc2

Souris

 

9L-Luc

Rats

Vessie

T24-Luc-Neo

Homme

 

MB49-Luc-mCh-Puro

Souris

Colon

COLO 205-Luc #2

Homme

 

HCT-116-Luc

Homme

 

HT-29-Luc

Homme

 

CT26.WT-luc-mCh-puro

Souris

 

MC38-NCI.TD1-luc-mCh-puro

Souris

Endometrial

KLE-Luc-mCh-Puro

Homme

Leucémie (LMA)

Kasumi-3-Luc-mCh-Puro

Homme

 

KG-1-Luc-mCh-Puro

Homme

 

MV-4-11-Luc-mCh-Puro

Homme

 

C1498-Luc-mCh-Puro

Souris

Leucémie [B-ALL]

NALM6-Luc-MCh-Puro

Homme

 

Reh (pMMP-Luc-Neo)

Homme

Leukemia [CML]

K-562-Luc2

Homme

Leukemia [erythro]

HEL 92.1.7-Luc-Neo

Homme

 

HEL-Luc-Neo

Homme

Leukemia [T-ALL]

DND-41-Luc-mCh-Puro

Homme

 

MOLT-4-Luc-MCh-Puro

Homme

Foie

BNL 1ME A.7R.1-Luc-mCh-Puro

Souris

 

Hep-55,1C-Luc-mCh-Puro

Souris

 

Hepa 1-6-Luc-mCh-Puro

Souris

Poumon

LL/2-Luc-M38

Souris

 

AB1-Luc-mCh-puro

Souris

Poumon [NSCLC]

A549-Luc-C8

Homme

 

HCC827-Luc-mCh-Puro

Homme

 

NCI-H125-Luc

Homme

 

NCI-H1703-Luc-mCh-Puro

Homme

 

NCI-H1975-Luc

Homme

 

NCI-H460-Luc2

Homme

 

PC-9-Luc-mCh-puro

Homme

Lymphome

EL4-Luc-mCh-Puro

Souris

Lymphome [cellules B]

A20-Luc2-Puro

Souris

Lymphome [de Burkitt]

Daudi-Luc-mCh-Puro

Homme

 

Raji-Luc

Homme

 

Ramos-Luc

Homme

Lymphoma [Diffuse Mixed]

SU-DHL-6-Luc-mCh-Puro

Homme

Lymphome [DLBCL]

OCI-Ly19-Luc-Neo

Homme

 

OCI-Ly3-Luc-mCh-Puro

Homme

 

OCI-Ly7-Luc-Neo

Homme

 

SU-DHL-4-Luc-mCh-Puro

Homme

 

Toledo-Luc-Neo

Homme

 

OCI-Ly1 R10-Luc-mCh-Puro

Homme

 

OCI-Ly1 R7-Luc-mCh-Puro

Homme

Mammaire/Sein

MCF7-Luc-mCh-Puro

Homme

 

MDA-MB-231-Luc-D3H1

Homme

 

E0771-Luc-mCh-Puro

Souris

 

MDA-MB-231-Luc-D3H2LN

Homme

 

EMT6-Luc-mCh-Puro

Souris

 

MX-1-Luc

Homme

 

4T1-Luc2-1A4

Souris

Mélanome

OCM-1-Luc-mCh-Puro

Homme

 

SK-MEL-28-Luc-mCh-Puro

Homme

 

B16-F10-Luc-G5

Souris

 

B16-F10-Luc2

Souris

 

Cloudman S91-Luc-mCh-Puro

Souris

 

YUMM1,7-Luc-mCh-Puro

Souris

Myélome

JJN-3-Luc-G418R

Homme

 

MM.1S (pMMP-Luc-Neo)

Homme

 

NCI-H929-Luc-mCh-Puro

Homme

 

5TGM1-Luc

Souris

 

J558-Luc-mCh-Puro

Souris

Ovarien

A2780-Luc

Homme

 

IGROV1-Luc-Mch-Puro

Homme

 

OVCAR-5-Luc-mCh-Puro

Homme

 

OVCAR-8-Luc-mCh-Puro

Homme

 

SK-OV-3-Luc-D3

Homme

 

ID8-Luc-mCh-Puro

Souris

 

NIH:OVCAR-3-Luc-mCh-Puro

Homme

Pancreatic

BxPC-3-Luc2

Homme

 

MIA PaCa-2-Luc

Homme

 

PANC-1-Luc

Homme

 

Pan02.TD1-Luc-mCh-Puro

Souris

Prostate

DU 145-Luc

Homme

 

PC-3-Luc

Homme

 

PC-3M-Luc-C6

Homme

Rénal

293-Luc-mCh-Puro

Homme

 

786-O-Luc-Neo (rescued)

Homme


Table 1: Luciferase-labeled cell lines

Contact us to request the full data set or to learn more about our luciferase enabling service and how it can be applied to your preclinical research.  


Références

1 Jessamy C. Tiffen, Charles G. Bailey, Cynthia Ng, John E.J. Rasko & Jeff Holst. Luciferase expression and bioluminescence does not affect tumor cell growth in vitro or in vivo. Molecular Cancer 2010; 9: 299.

2 Dan M. Close, Tingting Xu, Gary S. Sayler, Steven Ripp. In vivo bioluminescent imaging (BLI): noninvasive visualization and interrogation of biological processes in living animals. Sensors (Basel) 2011; 11(1): 180–206.Note: Please note that all animal care and use was conducted according to animal welfare regulations in an AAALAC-accredited facility with IACUC protocol review and approval.

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